JMJD2b active human
SIGMA/SRP0320 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)
Synonym: KDM4B; lysine (K)-specific demethylase 4B
Product Type: Chemical
SDS-PAGE JMJD2b active Human : Cat. No. SRP0320: Coommassie blue-stained SDS-PAGE analysis of JMJD2b. Lanes 1. 10 μg JMJD2b. 2. Protein Marker.
Chart JMJD2b active Human : Cat. No. SRP0320: Activity assay of JMJD2b Active Human.
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 83 kDa |
| NCBI accession no. | NM_015015   |
| packaging | pkg of 100 μg |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O94953 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | 0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection. |
| General description: | Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system. |
| Physical form: | Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol. |
| Preparation Note: | At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |