Ceramide Kinase FLAG tag active human
SIGMA/SRP0339 - recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)
Synonym: CERK; LK4; hCERK
Product Type: Chemical
SDS-PAGE Ceramide Kinase FLAG tag active Human : Cat. No. SRP0339: Coommassie blue-stained SDS-PAGE analysis of CERK. Lanes 1. 5.6 μg CERK. 2. Protein Marker.
Chart Ceramide Kinase FLAG tag active Human : Cat. No. SRP0339: Activity assay of Ceramide Kinase, FLAG tagged, Active Human.
| assay | ≥40% (SDS-PAGE) |
| biological source | human |
| concentration | 0.4 mg/mL |
| form | aqueous solution |
| mol wt | 60 kDa |
| NCBI accession no. | NM_022766   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells. |
| General description: | Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31. |
| General description: | Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system. |
| Other Notes: | One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥40% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |