Aurora Kinase A active human
SIGMA/SRP0354 - recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)
Synonym: ARK1; AURA; BTAK; STK6
CAS Number: 458560-40-2
Product Type: Chemical
SDS-PAGE Aurora Kinase A active Human : Cat. No. SRP0354: Coommassie blue-stained SDS-PAGE analysis of AuroraA. Lanes 1. 2 μg AuroraA. 2. Protein Marker.
Chart Aurora Kinase A active Human : Cat. No. SRP0354: Activity assay of Aurora Kinase A, Active Human.
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 50 kDa |
| NCBI accession no. | NM_003600   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | activity assay: suitable |
| inhibition assay: suitable | |
| UniProt accession no. | O14965 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | AURA/AURKA (aurora kinase A) modulates cell cycle checkpoints and plays important roles in centrosome division, cytoskeleton and cytokinesis. It sustains the integrity of chromosomes after DNA damage. The catalytic domain of AURKA possesses a serine/threonine kinase activity. |
| General description: | AURA/AURKA (aurora kinase A) is mapped to human chromosome 20q13.2. It is also termed as STK15 (serine/threonine kinase 15). This gene codes for a protein of 403 amino acids with regulatory and catalytic domains. AURA is overexpressed in several malignancies like invasive carcinomas of ovary, head, neck and breast. |
| General description: | Human Aurora kinase A (GenBank Accession No. NM_003600), amino acids 2- 403 with N-terminal HIS6-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Other Notes: | One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |