GSK3β active human
SIGMA/SRP0356 - recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)
Synonym: glycogen synthase kinase 3 beta
CAS Number: 443900-95-6
Product Type: Chemical
SDS-PAGE GSK3β active Human : Cat. No. SRP0356: Coommassie blue-stained SDS-PAGE analysis of GSK3beta. Lanes 1. 5 μg GSK3beta. 2. Protein Marker.
Chart GSK3β active Human : Cat. No. SRP0356: Activity assay of GSK3beta Active Human.
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 73 kDa |
| NCBI accession no. | NM_002093   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | ≥500 pmol/min-μg |
| storage temp. | −70°C |
| technique(s) | activity assay: suitable |
| inhibition assay: suitable | |
| UniProt accession no. | P49841 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple protein which are linked with Alzheimer’s disease (AD). |
| General description: | Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain. |
| General description: | Human GSK3beta GenBank Accession No. NM_002093), full length with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| activity | specific activity: ≥500 pmol/min-μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |