PLK1 Polo Box Domain active human
SIGMA/SRP0360 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: STPK13; polo-like kinase 1
Product Type: Chemical
SDS-PAGE PLK1 Polo Box Domain active Human : Cat. No. SRP0360: Coommassie blue-stained SDS-PAGE analysis of Plk1. Lanes 1. 4 μg Plk1. 2. Protein Marker.
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 2.9 mg/mL |
| form | aqueous solution |
| mol wt | 53 kDa |
| NCBI accession no. | NM_005030   |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P53350 |
| Application: | PLK1 Polo Box Domain active human has been used in the in vitro binding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQ |
| Biochem/physiol Actions: | Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors. |
| General description: | Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system. |
| General description: | Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |