mTOR/Raptor/MLST8 human
SIGMA/SRP0364 - recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)
Product Type: Chemical
SDS-PAGE mTOR/Raptor/MLST8 Human : Cat. No. SRP0364: Coommassie blue-stained SDS-PAGE analysis of enzyme complex. Lanes 1. 3 μg enzyme complex. 2. Protein Marker.
Chart mTOR/Raptor/MLST8 Human : Cat. No. SRP0364: Activity assay of mTOR/Raptor/MLST8 Human.
| application(s) | life science and biopharma |
| assay | ≥50% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | 137 kDa (mTOR) |
| 150 kDa (Raptor) | |
| 37 kDa (MLST8) | |
| NCBI accession no. | NM_004958 (mTOR)   |
| NM_020761 (Raptor) |
|
| NM_022372 (MLST8) |
|
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| solubility | water: soluble |
| storage temp. | −70°C |
| technique(s) | cell based assay: suitable |
| Application: | Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions. |
| Biochem/physiol Actions: | Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them. |
| General description: | This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥50% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |