PRMT6 active human
SIGMA/SRP0389 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: HRMT1L6; Histone-arginine N-methyltransferase 6; protein arginine N-methyltransferase 6
Product Type: Chemical
SDS-PAGE PRMT6 active Human : Cat. No. SRP0389: Coommassie blue-stained SDS-PAGE analysis of PRMT6. Lanes 1. 3 μg PRMT6. 2. Protein Marker.
Chart PRMT6 active Human : Cat. No. SRP0389: Activity assay of PRMT6 Active Human.
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 43 kDa |
| NCBI accession no. | NM_018137   |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q96LA8 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | Protein arginine methyltransferase 6 (PRMT6) catalyze the formation of asymmetric ω-NG,NG -dimethylarginine residues. In addition, PRMT6 show automethylation activity, which is unique among the members of PRMT family. PRMT6 mediates cigarette smoke extract (CSE)-triggered apoptosis and inflammation via di-methylation H3 arginine 2 (H3R2me2a) in human umbilical vein endothelial cells (HUVECs). PRMT6 plays a crucial role in various physiological processes such as transcriptional regulation, DNA repair and human immunodeficiency virus pathogenesis. Upregulated expression of the enzyme might be favorable for tumor growth and progression. Overexpression of PRMT6 has been observed in various type of malignancies including breast, cervix, bladder, prostate and lung cancer. |
| General description: | Protein arginine methyltransferase 6 (PRMT6) is a 41.9kDa protein with 375 amino acids. It is encoded by the gene mapped to human chromosome 1. It belongs to type I enzyme, which is a subclass of PRMT family. PRMT6 is localized exclusively in nucleus. Human PRMT6 (GenBank Accession No. NM_018137), amino-acids 2-375 (end), with N-terminal His-tag, MW=43kDa, expressed in Escherichia coli. |
| Physical form: | Formulated in 200 mM imidazole, 20% glycerol, and 3 mM DTT. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |