SETD2 active human
SIGMA/SRP0395 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: HYPB; SET domain containing 2; SET2
Product Type: Chemical
SDS-PAGE SETD2 active Human : Cat. No. SRP0395: Coommassie blue-stained SDS-PAGE analysis of SETD2. Lanes 1. 4.4 μg SETD2. 2. Protein Marker.
Chart SETD2 active Human : Cat. No. SRP0395: Activity assay of SETD2 Active Human.
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 60.9 kDa |
| NCBI accession no. | NM_014159   |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q9BYW2 |
| Biochem/physiol Actions: | SET domain containing 2 (SETD2) plays a vital role in trimethylation of the histone mark H3K36 (histone 3 lysine 36). Mutation of this tumor suppressor gene (TSG) is associated with the pathogenesis of various cancers including, clear cell renal cell carcinoma (cRCC), breast cancer, lung cancer, acute lymphoblastic leukemia(ALL), and glioma. In addition, SETD2 gene is also involved in the development of Huntington′s disease (HD). SETD2 plays an important role in the transcriptional elongation by maintaining chromatin structure through association with hyperphosphorylated RNA polymerase II (POLR2A). SETD2 interacts with p53 and act as a tumor suppressor in breast cancer cells. SETD2 is implicated in DNA double-strand break (DSB) exclusion and homologous recombination (HR) repair in human cells. SETD2 in HeLa cells alters nucleosome organization via decreasing the recruitment of the FACT (facilitates chromatin transcription) complex to active genes. |
| General description: | SET domain containing 2 (SETD2) is a histone methyltransferase gene and is mapped on human chromosome 3p21.31, a region associated with growth arrest of cancer cells. Human SETD2/pGEX6P1 (GenBank Accession No. NM_014159), amino acids 1418-1714 with N-terminal GST-tag, MW= 60.9kDa, expressed in an Escherichia coli expression system. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |