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DNMT3A/DNMT3L active human

SIGMA/SRP0396 - recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SRP0396-10UG 10 µg
 $687.00
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SDS-PAGE DNMT3A/DNMT3L active Human : Cat. No. SRP0396: Coommassie blue-stained SDS-PAGE analysis of DMNT3A/DNMT3L. Lanes 1. 3 μg DMNT3A/DNMT3L. 2. Protein Marker.
Chart DNMT3A/DNMT3L active Human : Cat. No. SRP0396: Activity assay of DNMT3A/DNMT3L Active Human.

 

assay ≥90% (SDS-PAGE)
biological source human
form aqueous solution
mol wt 34 kDa (MLL1)
  52 kDa (DNMT3L)
NCBI accession no. NM_013369 (DNMT3L) 
  NM_175629 (DNMT3A) 
packaging pkg of 10 μg
recombinant expressed in baculovirus infected Sf9 cells
shipped in dry ice
storage temp. −70°C
UniProt accession no. Q9Y6K1 
Application: DNMT3A/DNMT3L active human has been used in in vitro methylation assay and REMSA (RNA electrophoretic mobility shift assay) to study role of ecRNAs (extra coding RNAs) in DNA methylation.
Biochem/physiol Actions: DNMT3A (DNA (cytosine-5)-methyltransferase 3A) is a DNA methyltransferase. It is responsible for the methylation of the cytosine residue in 5′-C-phosphate-G-3′ (CpG) dinucleotides. DNMT3A plays a significant role in immunoregulation (adaptive as well as innate immune responses). It is also the most commonly mutated gene in hematologic malignancies. DNMT3A is a commonly mutated gene in acute myeloid leukemia and is responsible for an unfavorable prognosis.
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) is mainly responsible for the stimulation of DNA methylation. It is a non-catalytic accessory factor. It helps the DNA methylation machinery to be placed on properly chromatinized DNA regions. It also helps the poorly methylated area, thereby providing uniform methylation patterns. Mutations in the DNMT3L gene might be associated with azoospermia susceptibility.
General description: The DNMT3A (DNA (cytosine-5)-methyltransferase 3A) gene is mapped to human chromosome 2p23. The encoded protein has 2 isoforms, DNMT3A1 and DNMT3A2. DNMT3A1 is widely expressed and DNMT3A2 is mainly present in embryonic stem cells (ovaries and testes).
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) belongs to the Dnmt3 family. It is unable to methylate DNA. The protein has a cysteine-rich region containing a novel-type zinc finger domain. The gene is mapped to human chromosome 21q22.3.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (SDS-PAGE)
Storage Temp. −70°C
UNSPSC 12352200

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