Histone Octamer full length human
SIGMA/SRP0408 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Product Type: Chemical
SDS-PAGE Histone Octamer full length Human : Cat. No. SRP0408: Coommassie blue-stained SDS-PAGE analysis of Histone Octamer full length. Lanes 1. no load amount on DS Histone Octamer full length. 2. Protein Marker.
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 113.8 kDa |
| NCBI accession no. | NM_003528   |
| NM_003532 |
|
| NM_003548 |
|
| NM_033445 |
|
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| Biochem/physiol Actions: | The nuclear DNA in eukaryotes is found to be associated with histones to form a compact complex called nucleosome. Histones neutralize the electrostatic nature of DNA and function as scaffolding proteins. Each core nucleosome contains two copies each of the core histones H2A, H2B, H3, and H4 to form an octameric complex. This octameric complex contains a central (H3-H4)2 tetramer flanked on both sides with H2A-H2B dimers. The octamer complex function in various stages of chromosome function, chromatin assembly and nucleosome formation. The histone dimer-tetramer interactions are also important in RNA transcription. |
| General description: | Human recombinant histone octamer consisting of 2 molecules each of histones H2A (GenBank Accession No. NM_033445) amino acids 2-130(end) with a N-terminal His-tag, H2B (GenBank Accession No. NM_003528) amino acids 2-126(end) with a N-terminal His-tag, H3 (GenBank Accession No. NM_003532) amino acids 2-137(end) with a N-terminal His-tag, and H4 (GenBank Accession No. NM_003548) amino acids 2-103(end) with a N-terminal His-tag, expressed in an E. coli expression system. |
| Risk Statements | 52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |