ALOX15 active human
SIGMA/SRP0410 - recombinant, expressed in baculovirus infected Sf9 cells, ≥60% (SDS-PAGE)
Synonym: 15LOX-1; arachidonate 15-lipoxygenase
Product Type: Chemical
SDS-PAGE ALOX15 active Human : Cat. No. SRP0410: Coommassie blue-stained SDS-PAGE analysis of ALOX-15. Lanes 1. 1.8 μg ALOX-15. 2. Protein Marker.
Chart ALOX15 active Human : Cat. No. SRP0410: Activity assay of ALOX15 Active Human.
| assay | ≥60% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 75 kDa |
| NCBI accession no. | NM_001140   |
| packaging | pkg of 20 μg |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P16050 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | ALOX15 (arachidonate 15-lipoxygenase) is involved in homeostatic as well as pathological responses. ALOX15 is responsible for the oxidation of unsaturated fatty acids (at the 15 position) to active hydroperoxy and epoxy metabolites. It is also responsible for the conversion of linoleic acid to anti-tumor 13-S-hydroxyoctadecadienoic acid. ALOX15 is required for IL-4 (interleukin-4)/IL-13-med |
| General description: | The gene ALOX15 (arachidonate 15-lipoxygenase) is mapped to human chromosome 17p13. It is mainly present in the epithelial cells in the upper airways, reticulocytes, eosinophils and macrophages. ALOX15 belongs to the dioxygenases family. It is an IL-4 (interleukin 4)/IL-13 target gene. ALOX-15, also known as 15LOX-1, ω-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140) amino acids 2- 662 (end) with N-terminal FLAG-tag, MW = 75kDa, expressed in Sf9 insect cells via a Baculovirus expression system. |
| Physical form: | Formulated in Tris-Buffered Saline (TBS), pH 7.4, containing 1 mM Fe(NH4)2(SO4)2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥60% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |