PKM1 active human
SIGMA/SRP0415 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: OIP3; PK3; PKM2; TCB; pyruvate kinase, muscle
CAS Number: 1644286-11-2
Product Type: Chemical
SDS-PAGE PKM1 active Human: Cat. No. SRP0415: Coommassie blue-stained SDS-PAGE analysis of PKM1. Lanes 1. 13.5 μg PKM1. 2. Protein Marker.
Chart PKM1 active Human: Cat. No. SRP0415: Activity assay of PKM1 Active Human.
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| concentration | 1.8 mg/mL |
| form | aqueous solution |
| mol wt | 59 kDa |
| NCBI accession no. | NM_182470   |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P14618 |
| Application: | PKM1active human has been used in a cell-based kinase assay to study the ability ofa compound to inhibit the kinase activity of PKM1. It has also been used inoxidative stress experiments on PKM2. |
| Biochem/physiol Actions: | Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction. |
| General description: | Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |