T4 Beta-glucosyltransferase
SIGMA/SRP0416 - recombinant, expressed in E. coli, ≥83% (SDS-PAGE)
Synonym: T4-phage beta-
Product Type: Chemical
assay | ≥83% (SDS-PAGE) |
biological source | Escherichia coli |
form | aqueous solution |
mol wt | 41.6 kDa |
NCBI accession no. | NP_049658 |
packaging | pkg of 100 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage temp. | −70°C |
UniProt accession no. | P04547 |
Application: | Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. |
Biochem/physiol Actions: | T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme. |
General description: | T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli. |
Physical form: | Formulated in 200 mM imidazole and 20% glycerol. |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H315 - H319 - H360D |
Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | T |
Risk Statements | 61 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥83% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |