T4 Beta-glucosyltransferase
SIGMA/SRP0416 - recombinant, expressed in E. coli, ≥83% (SDS-PAGE)
Synonym: T4-phage beta-
Product Type: Chemical
SDS-PAGE T4 Beta-glucosyltransferase: Cat. No. SRP0416: Coommassie blue-stained SDS-PAGE analysis of β-glucosyltransferase. Lanes 1. 8 μg β-glucosyltransferase. 2. Protein Marker.
| assay | ≥83% (SDS-PAGE) |
| biological source | Escherichia coli |
| form | aqueous solution |
| mol wt | 41.6 kDa |
| NCBI accession no. | NP_049658   |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P04547 |
| Application: | Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. |
| Biochem/physiol Actions: | T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme. |
| General description: | T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli. |
| Physical form: | Formulated in 200 mM imidazole and 20% glycerol. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥83% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |