BirA
SIGMA/SRP0417 - recombinant, expressed in E. coli, ≥65% (SDS-PAGE)
Product Type: Chemical
assay | ≥65% (SDS-PAGE) |
form | aqueous solution |
mol wt | 37 kDa |
NCBI accession no. | AP012306 |
packaging | pkg of 100 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage temp. | −70°C |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Biochem/physiol Actions: | Biotin ligase BirA controls biotin synthesis in Escherichia coli. It sends biotin to metabolism. As a homodimer, it negatively controls the biotin synthetic operon. BirA also catalyzes post-translational biotinylation, by associating with biotin acceptor protein of acetyl-coenzyme A carboxylase. It can biotinylate human histones. |
General description: | Biotin ligase BirA (GenBank Accession No. AP012306 (3602298-3603260)) amino acids 2 – 321 (end) with His-FLAG-tag, MW = 37.1 kDa, expressed in an Escherichia coli cell expression system. |
Physical form: | Formulated in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% Glycerol. |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H315 - H319 - H360D |
Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | T |
Risk Statements | 61 |
Safety Statements | 53-35-36/37 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥65% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |