BirA
SIGMA/SRP0417 - recombinant, expressed in E. coli, ≥65% (SDS-PAGE)
Synonym: BirA Biotin Ligase
Product Type: Chemical
SDS-PAGE BirA: Cat. No. SRP0417: Coommassie blue-stained SDS-PAGE analysis of BirA. Lanes 1. 4 μg BirA. 2. Protein Marker.
| assay | ≥65% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 37 kDa |
| NCBI accession no. | AP012306   |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | Biotin ligase BirA controls biotin synthesis in Escherichia coli. It sends biotin to metabolism. As a homodimer, it negatively controls the biotin synthetic operon. BirA also catalyzes post-translational biotinylation, by associating with biotin acceptor protein of acetyl-coenzyme A carboxylase. It can biotinylate human histones. |
| General description: | Biotin ligase BirA (GenBank Accession No. AP012306 (3602298-3603260)) amino acids 2 – 321 (end) with His-FLAG-tag, MW = 37.1 kDa, expressed in an Escherichia coli cell expression system. |
| Physical form: | Formulated in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% Glycerol. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-35-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥65% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |