RNA Polymerase II, C-terminal human

SIGMA/SRP2013 - recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Synonym: POLRA; RPB1; RPO2; RPOL2; hRPB220

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-SRP2013-10UG	10 µg	$472.00 1/EA		Add To Favorites

Immunoblotting RNA Polymerase II, C-terminal Cat. No. SRP2013: RNA Polymerase II, C-terminal was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).

Properties
Descriptions
Safety Info.
Basic Data

assay	≥85% (SDS-PAGE)
biological source	human
color	clear colorless
concentration	400 μg/mL
form	frozen liquid
mol wt	~42.2 kDa
NCBI accession no.	NM_000937
packaging	pkg of 10 μg
recombinant	expressed in E. coli
shipped in	dry ice
storage condition	avoid repeated freeze/thaw cycles
storage temp.	−70°C
UniProt accession no.	P24928

Biochem/physiol Actions:	The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo, designated IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongating complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.
General description:	RNA polymerase II (eukaryotic) contains around 10 subunits with a combined molecular weight of 500,000Da. The subunit structure of this enzyme is conserved amongst eukaryotes, and this subunits structure shares similarities with that of RNA pol I and III. The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms.
Physical form:	Clear and colorless frozen liquid solution
Preparation Note:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥85% (SDS-PAGE)
Storage Temp.	−70°C
UNSPSC	12352202

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