GAL4 [(1-147) - AH] from Saccharomyces cerevisiae
SIGMA/SRP2016 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: Gal81
Product Type: Chemical
Immunoblotting GAL4 [(1-147) - AH] Cat. No. SRP2016: GAL4 [(1-147) - AH] was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥80% (SDS-PAGE) |
| biological source | Saccharomyces cerevisiae |
| color | colorless to clear |
| concentration | 300 μg/mL |
| form | frozen liquid |
| mol wt | ~18.6 kDa |
| NCBI accession no. | NM_001184062   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| technique(s) | electrophoretic mobility shift assay: suitable |
| UniProt accession no. | P04386 |
| Biochem/physiol Actions: | Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |