GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus
SIGMA/SRP2018 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: E1A
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
biological source | Saccharomyces cerevisiae |
canine | |
color | colorless to clear |
concentration | 100 μg/mL |
form | frozen liquid |
mol wt | ~33 kDa |
NCBI accession no. | J04368 |
NM_001184062 | |
packaging | pkg of 4 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
technique(s) | electrophoretic mobility shift assay: suitable |
UniProt accession no. | P04386 |
Biochem/physiol Actions: | The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |