TR, β 1 isoform human
SIGMA/SRP2020 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: ERBA-beta; GRTH; NR1A2; THR1; THRB1
Product Type: Chemical
Immunoblotting TR, β 1 isoform Cat. No. SRP2020: TR, β 1 isoform was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 500 μg/mL |
| form | frozen liquid |
| mol wt | ~53 kDa |
| NCBI accession no. | NM_000461   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P10828 |
| Biochem/physiol Actions: | Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approximately 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approximately 250 amino acids. The amino-terminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TR α and TR β isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TR β gene generates two isoforms, TR β1 and TR β2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and transcription factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein–associated factor (PCAF), and CREB binding protein (CBP). |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |