Dr1 (NC2b, 19 kDa) human
SIGMA/SRP2021 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: NC2; NC2-beta
Product Type: Chemical
Immunoblotting Dr1 (NC2b, 19 kDa) Cat. No. SRP2021: Dr1 (NC2b, 19 kDa) was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| color | colorless to clear |
| concentration | 450 μg/mL |
| form | frozen liquid |
| mol wt | ~20.6 kDa |
| NCBI accession no. | NM_001938   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q01658 |
| Biochem/physiol Actions: | Dr1 is a general negative regulator of class II and class III gene expression. It binds to the basic repeat domain of TBP on promoter DNA and can prevent the RNA polymerase II holoenzyme, or its TFIIB and/or TFIIA subunits, from assembling into an initiation complex. Dr1 is phosphorylated in vivo and this modification affects its interaction with TBP. In addition, Dr1 interacts with the hyperphosphorylated form of Pol II and with the repression domain of the AREB6 repressor. Dr1 forms a heterodimer complex with DRAP1 through its histone fold domain. More recently it was discovered that Dr1-DRAP1 is a bi-functional basal transcription factor that differentially regulates gene transcription through DPE (downstream promoter elements) or TATA box motifs. It can stimulate transcription in vitro from Drosophila promoters containing DPEs, whereas it represses transcription from TATA-containing promoters. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |