TFIIA, reconstituted human
SIGMA/SRP2028 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: MGC129969; TF2A1; TF2A2; TFIIA; TFIIA
Product Type: Chemical
Immunoblotting TFIIA, reconstituted Cat. No. SRP2028: TFIIA, reconstituted was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 150 μg/mL |
| form | frozen liquid |
| mol wt | 66 kDa |
| NCBI accession no. | NM_004492   |
| NM_015859 |
|
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P52655 |
| Biochem/physiol Actions: | The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α subunit), 19 kDa (β subunit) and 12 kDa (γ subunit). The α and β subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ subunit), retains native TFIIA activity. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |