Sp1 GC-box binding protein human
SIGMA/SRP2030 - recombinant, expressed in insect cells, ≥75% (SDS-PAGE)
Product Type: Chemical
Immunoblotting Sp1 GC-box binding protein Cat. No. SRP2030: Sp1 GC-box binding protein was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥75% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 200 μg/mL |
| form | frozen liquid |
| mol wt | ~81.6 kDa |
| NCBI accession no. | NM_138473   |
| packaging | pkg of 5 μg |
| recombinant | expressed in insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| technique(s) | electrophoretic mobility shift assay: suitable |
| UniProt accession no. | P08047 |
| Application: | Sp1 transcription factor has been used as a standard in electrophoretic mobility shift assay. |
| Biochem/physiol Actions: | Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well-characterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥75% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |