CTF1 human
SIGMA/SRP2031 - recombinant, expressed in insect cells, ≥70% (SDS-PAGE)
Synonym: CTF; CTF/NFI; NF-1; NF-I
Product Type: Chemical
Immunoblotting CTF1 Cat. No. SRP2031: CTF1 was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| color | colorless to clear |
| concentration | 500 μg/mL |
| form | frozen liquid |
| mol wt | ~56.4 kDa |
| NCBI accession no. | X12492   |
| packaging | pkg of 5 μg |
| recombinant | expressed in insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q16619 |
| Biochem/physiol Actions: | The CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator. It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes. Cloning and functional characterization of the protein revealed that CTF1 contains an N-terminal DNA binding domain and a C-terminal proline-rich activation domain. CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes, with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene, as well as other receptor proteins to synergize hormone receptor-responsive activation. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |