Topo I (N1-197) (NTD) human
SIGMA/SRP2034 - recombinant, expressed in insect cells, ≥85% (SDS-PAGE)
Synonym: TOPI
Product Type: Chemical
assay | ≥85% (SDS-PAGE) |
biological source | human |
color | clear colorless |
concentration | 1000 μg/mL |
form | frozen liquid |
mol wt | ~25.3 kDa |
NCBI accession no. | NM_003286 |
packaging | pkg of 5 μg |
recombinant | expressed in insect cells |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | P11387 |
Biochem/physiol Actions: | Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The N-terminal 214 amino acids of topoisomerase I comprise a highly charged N-terminal domain (NTD) involved in protein-protein interactions with a number of cellular proteins, including SV40 T antigen, nucleolin, SR proteins, p53 and other transcription factors. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥85% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352202 |