Thyroid Hormone receptor,α1 human
SIGMA/SRP2046 - recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
Synonym: AR7; EAR7; ERB-T-1; ERBA; ERBA1; MGC000261; MGC43240; NR1A1; THRA1; c-ERBA-1
Product Type: Chemical
assay | ≥80% (SDS-PAGE) |
biological source | human |
color | clear colorless |
concentration | 500 μg/mL |
form | frozen liquid |
mol wt | ~56.5 kDa |
NCBI accession no. | NM_003250 ![]() ![]() |
packaging | pkg of 5 μg |
recombinant | expressed in insect cells |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | P10827 ![]() ![]() |
Biochem/physiol Actions: | Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (α) the central, highly conserved, DNA-binding domain (DBD) of approximately 66 amino acids, and (β) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approximately 250 amino acids. The amino-terminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TRα and TRβ isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TRβ gene generates two isoforms, TRβ1 and TRβ2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and transcription factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein–associated factor (PCAF), and CREB binding protein (CBP). |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥80% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |