HMG1, native from calf thymus
SIGMA/SRP2051 - ≥85% (SDS-PAGE)
Product Type: Chemical
Immunoblotting HMG1 Cat. No. SRP2051: HMG1 was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥85% (SDS-PAGE) |
| biological source | bovine (calf) thymus |
| color | colorless to clear |
| concentration | 900 μg/mL |
| form | frozen liquid |
| mol wt | ~24.9 kDa |
| NCBI accession no. | X12796   |
| packaging | pkg of 10 μg |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| technique(s) | membrane array: suitable |
| UniProt accession no. | P10103 |
| Biochem/physiol Actions: | High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by directly contacting with distinct cellular proteins. HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |