SRC1, His tagged, TEV site, human
SIGMA/SRP2068 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: F-SRC-1; KAT13A; MGC129719; MGC129720; RIP160; SRC1; bHLHe42; bHLHe74
Product Type: Chemical
assay | ≥80% (SDS-PAGE) |
biological source | human |
color | clear colorless |
concentration | 400 μg/mL |
form | frozen liquid |
mol wt | ~19.5 kDa |
NCBI accession no. | NM_003743 |
packaging | pkg of 10 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | Q15788 |
Biochem/physiol Actions: | Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family. SRC1 has been shown to be over expressed in some cancers. SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction. The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptorsand have been previously used in assays detecting ligand-dependent receptor-cofactor interactions. This protein contains the Tobacco Etch virus (TEV) protease site and can be cleaved using acTEV to yield untagged SRC1 RID. This protein will be useful for assays where a native untagged protein is desired. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥80% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |