WT-1 (+KTS) human
SIGMA/SRP2082 - recombinant, expressed in insect cells, ≥60% (SDS-PAGE)
Product Type: Chemical
assay | ≥60% (SDS-PAGE) |
biological source | human |
color | clear colorless |
concentration | 150 μg/mL |
form | frozen liquid |
NCBI accession no. | NM_024425 ![]() ![]() |
packaging | pkg of 5 μg |
recombinant | expressed in insect cells |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | P19544 ![]() ![]() |
Biochem/physiol Actions: | WT-1, the product of Wilms’ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms’ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥60% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |