C-jun, proto oncogene human

SIGMA/SRP2088 - recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

Synonym: AP-1; AP1; c-Jun

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-SRP2088-5UG	5 µg	$575.00 1/EA		Add To Favorites

Immunoblotting C-jun, proto oncogene Cat. No. SRP2088: C-jun, proto oncogene was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).

Properties
Descriptions
Safety Info.
Basic Data

assay	≥80% (SDS-PAGE)
biological source	human
color	clear
	colorless
concentration	250 μg/mL
form	frozen liquid
mol wt	~37.5 kDa
NCBI accession no.	NM_002228
packaging	pkg of 5 μg
recombinant	expressed in insect cells
shipped in	dry ice
storage condition	avoid repeated freeze/thaw cycles
storage temp.	−70°C
UniProt accession no.	P05412

Biochem/physiol Actions:	The transcription factor AP-1 (activator protein-1) is involved in cellular proliferation, transformation and death. AP-1 and nuclear factor B (NF- B) can be specifically targeted to prevent cancer induction in mouse models. AP-1 can be produced by 18 different dimeric combinations of proteins from the Jun (c-Jun, JunB and JunD) and Fos (c-Fos, FosB, Fra-1 and Fra-2) families, including Jun homodimers and Jun-Fos heterodimers. The Jun and Fos proteins contain a basic-region leucine zipper (bZIP) domain, and are capable of binding to other bZIP proteins including those from the ATF, MAF, CNC and C/EBP (CCAAT/enhancer-binding protein) subfamilies (5]) Jun-Jun and Jun-Fos dimers bind with highest affinity to the 12-O-tetradecanoylphorbol-13-acetate (TPA) response element (TRE) [TGA(C/G)TCA], although many other ′AP-1-like sites′ have been reported. Binding to any of these sites can be tissue-specific, or affected by neighboring sequences, and dependent upon interactions with other transcription factors or cofactors. Jun and Fos proteins can also dimerize with other bZIP proteins, allowing them to target other DNA binding sites, such as the cAMP response element (CRE), the antioxidant response elements (ARE), and half-sites composed of half of a TRE site and half of a MAF- or CNC-binding site. In addition, AP-1 proteins can interact with other proteins, including the p65 subunit of NF- B, CBP (CRE-binding-protein-binding protein) (p300), SMAD-3 and -4, and the retinoblastoma protein (see 5 for a more complete list), further increasing the combinatorial potential of Jun and Fos proteins. AP-1 regulates a variety of cellular processes, including proliferation, differentiation and apoptosis, and contributes to both basal and stimulus-activated gene expression. It is activated by growth factors, hormones, stress, cytokines, ROS and ultraviolet radiation Activation occurs both transcriptionally and post-translationally, and is signaled predominantly through the mitogen-activated protein kinase (MAPK) cascade. The combinatorial diversity of AP-1 proteins and other interacting factors appears to influence how specific cell types respond to a stimulus. The growth-promoting activity of c-Jun is mediated by repression of tumor suppressors, as well as up-regulation of positive cell cycle regulators. Mostly, c-Jun is a positive regulator of cell proliferation, whereas JunB has the converse effect.
Physical form:	Clear and colorless frozen liquid solution
Preparation Note:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥80% (SDS-PAGE)
Storage Temp.	−70°C
UNSPSC	12352200

Choose a favorite list for this item:

Catalog Number	Description	Price

		$

Please choose a list :

Continue Shopping

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.