FGF-1 human
SIGMA/SRP2091 - recombinant, expressed in insect cells, ≥85% (SDS-PAGE)
Synonym: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-alpha; FGFA; GLIO703; HBGF1
Product Type: Chemical
Immunoblotting FGF-1 Cat. No. SRP2091: FGF-1 was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥85% (SDS-PAGE) |
| biological source | human |
| color | colorless to clear |
| concentration | 500 μg/mL |
| form | frozen liquid |
| mol wt | ~19.2 kDa |
| NCBI accession no. | NM_000800   |
| packaging | pkg of 5 μg |
| recombinant | expressed in insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P05230 |
| Application: | FGF-1 (fibroblast growth factor 1) has been used as a growth factor to study the effect of FGF1 on early branching events in isolated endoderm obtained from embryonic mouse lung. It has also been used to study the neuroprotection effect of FGF1 on cerebellar granule neurons obtained from rats. |
| Biochem/physiol Actions: | Acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) are ubiquitous cytokines found in many tissues. They have effects on multiple cell types derived from mesoderm and neuroectoderm, including endothelial cells. FGF proteins are small peptides of 155 to 268 amino acid residues. The degree of sequence identity between different family members is 30-60 % in a "central domain" of approx. 120 amino acids. This domain confers to FGFs a common tertiary structure and the ability to bind to heparin. Secreted FGFs signal to target cells by binding and activating cell-surface tyrosine kinase FGF receptors (FGFRs; 6, 7). The function of FGFs and FGFRs during embryonic development and adult physiology has been addressed by gain- and loss-of-function experiments in several animal model organisms. These studies have shown that FGFs act as key regulators of developmental events. |
| Biochem/physiol Actions: | The protein fibroblast growth factor 1, functions intracellularly to protect cells against stress. The protein functions as an angiogenic factor that participates in tissue repair, carcinogenesis, and maintenance of vasculature stability. Its expression is found to be upregulated in in patients with rheumatic arthritis. It serves as a prognostic biomarker in several cancers and a potential therapeutic target for patients with non-small cell lung cancer. |
| General description: | The gene FGF1 (fibroblast growth factor 1) encodes a member of the fibroblast growth factor (FGF) family. It encodes a pro-angiogenic protein that is ubiquitously expressed and is secreted via transmembrane translocation from the cells through a nonclassical secretory pathway in response to stress. It has the ability to permeate membranes composed of acidic phospholipids. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |