SV40 T-antigen from simian virus
SIGMA/SRP2093 - recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
Product Type: Chemical
assay | ≥80% (SDS-PAGE) |
biological source | simian virus |
color | clear colorless |
concentration | 400 μg/mL |
form | frozen liquid |
mol wt | ~83.3 kDa |
NCBI accession no. | AAG39214.1 ![]() ![]() |
packaging | pkg of 5 μg |
recombinant | expressed in insect cells |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | P03070 ![]() ![]() |
Biochem/physiol Actions: | The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively. |
Biochem/physiol Actions: | The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥80% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |