SV40 T-antigen from simian virus
SIGMA/SRP2093 - recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
Product Type: Chemical
Immunoblotting SV40 T-antigen Cat. No. SRP2093: SV40 T-antigen was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥80% (SDS-PAGE) |
| biological source | simian virus |
| color | clear colorless |
| concentration | 400 μg/mL |
| form | frozen liquid |
| mol wt | ~83.3 kDa |
| NCBI accession no. | AAG39214.1   |
| packaging | pkg of 5 μg |
| recombinant | expressed in insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P03070 |
| Biochem/physiol Actions: | The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively. |
| Biochem/physiol Actions: | The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |