TNFα, low endotoxin, His tagged human
SIGMA/SRP2102 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: DIF; TNF-alpha; TNFA; TNFSF2
Product Type: Chemical
Immunoblotting TNFα, low endotoxin, His tagged Cat. No. SRP2102: TNFα, low endotoxin, His tagged was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
TNF alpha Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 1250 μg/mL |
| form | frozen liquid |
| mol wt | ~21 kDa |
| NCBI accession no. | NM_000594   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P01375 |
| Biochem/physiol Actions: | Tumor necrosis factor α (TNFα or TNFSF2) is produced by many different cell types including activated monocytes, fibroblasts, endothelial cells, macrophages, T-cells, B-lymphocytes, granulocytes, smooth muscle cells, eosinophilis, chondrocytes and other cell types. It functions as a cytokine involved in cell proliferation, differentiation, apoptosis, lipid metabolism and coagulation. High level of TNFα leads to the development of inflammatory responses that are hallmarks of many diseases including autoimmune diseases, insulin resistance and cancer. The active form of TNFα (18 kDa) is generated by the cleavage of an Ala-Val bond between residues 76-77 of the precursor protein. |
| General description: | Tumor necrosis factor α (TNFα or TNFSF2) is encoded by the gene mapped to human chromosome 6p21.33. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |