Arginine Splicing Factor, His tagged human
SIGMA/SRP2104 - recombinant, expressed in insect cells, ≥85% (SDS-PAGE)
Synonym: ASF; FLJ53078; MGC5228; SF2; SF2p33; SFRS1; SRp30a
Product Type: Chemical
assay | ≥85% (SDS-PAGE) |
biological source | human |
color | clear |
colorless | |
concentration | 350 μg/mL |
form | frozen liquid |
mol wt | ~29.3 kDa |
NCBI accession no. | NM_006924 |
packaging | pkg of 5 μg |
recombinant | expressed in insect cells |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | Q07955 |
Biochem/physiol Actions: | ASF also known as SF2 is a member of the SR protein family. It is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating transcription and pre-mRNA splicing. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥85% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352202 |