TFIIF, Rap30 subunit, GST tagged human
SIGMA/SRP2114 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: BTF4; RAP30; TF2F2; TFIiF
Product Type: Chemical
Immunoblotting TFIIF, Rap30 subunit, GST tagged Cat. No. SRP2114: TFIIF, Rap30 subunit, GST tagged was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 900 μg/mL |
| form | frozen liquid |
| mol wt | ~52.9 kDa |
| NCBI accession no. | NM_004128   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P13984 |
| Biochem/physiol Actions: | The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP30) subunits as a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E coli factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |