RNA Polymerase II, p15.6 subunit, GST tagged human
SIGMA/SRP2116 - recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym: HRBP14.4; POLRF; RPABC2; RPB14.4; RPB6
Product Type: Chemical
Immunoblotting RNA Polymerase II, p15.6 subunit, GST tagged Cat. No. SRP2116: RNA Polymerase II, p15.6 subunit, GST tagged was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥85% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 200 μg/mL |
| form | frozen liquid |
| mol wt | ~40 kDa |
| NCBI accession no. | NM_021974   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P61218 |
| Biochem/physiol Actions: | hRPB6 is a highly conserved subunit shared by all three RNA polymerases, consisting of 142 amino acid residues. The gene for yeast RPB6 is essential for cell viability and homologues of this subunit exist in archaeal and some viral RNA polymerases. For example the bacterial w (the fifth subunit of the bacterial RNAP core enzyme) and eukaryotic RPB6 are structural homologs. RPB6 has been shown to promote pol II complex assembly, and/or increase its stability, through specific interactions with the largest subunit of RNA pol II. In addition, RPB6 was found to make contact with three small subunits, RPB5, RPB7, and RPB8 and to play a role in the interaction between RNA polymerase II and the transcription elongation factor TFIIS. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |