RNA Polymerase II, RPB10 subunit, GST tagged human
SIGMA/SRP2118 - recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym: RBP10; RPABC5; RPB10; RPB10beta; RPB7.6; hRPB7.6; hsRPB10b
Product Type: Chemical
assay | ≥85% (SDS-PAGE) |
biological source | human |
color | clear colorless |
concentration | 300 μg/mL |
form | frozen liquid |
mol wt | ~33.6 kDa |
NCBI accession no. | NM_021128 ![]() ![]() |
packaging | pkg of 10 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | P62875 ![]() ![]() |
Biochem/physiol Actions: | The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. It is a zinc-binding protein with an atypical CX2CXnCC metal binding motif. RPB10 participates in protein-protein interactions with additional components of the polymerase holoenzyme. Experimentally, the binding of RPB10 to an RPB3-RPB11 (or RPAC40-RPAC19 in RNAPI/III) heterodimer is well characterized and persists in both eukaryal and archaeal RNAPs. Binding of RPB10 to this a2-like heterodimer through zinc-mediated hemi-coordination suggests an early role in holoenzyme assembly since the formation of the α2-complex is the first step in the assembly of the prokaryotic RNAPs. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥85% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |