RNA Polymerase II, RPB12 subunit, GST tagged human
SIGMA/SRP2119 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: ABC10-alpha; RPABC4; RPB10alpha; RPB12; RPB7.0; hRPB7.0; hsRPB10a
Product Type: Chemical
Immunoblotting RNA Polymerase II, RPB12 subunit, GST tagged Cat. No. SRP2119: RNA Polymerase II, RPB12 subunit, GST tagged was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 100 μg/mL |
| form | frozen liquid |
| mol wt | ~33 kDa |
| NCBI accession no. | NM_005034   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P53803 |
| Biochem/physiol Actions: | RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability. RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart. |
| Biochem/physiol Actions: | RPB12/RPC10 (RNA polymerase II subunit K) is required for growth in Saccharomyces cerevisiae. The carboxy-terminal domain (CTD) of RNAP II plays a major role in recruiting many of these activities to the transcriptional machinery. |
| General description: | POLR2K (RNA polymerase II subunit K) is located on human chromosome 8q22. Rpb12 is the eukaryotic subunit that is also called as ABC10α. Rpb12 is the general subunit of all three eukaryotic RNA polymerases. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |