RNA Polymerase II, C-terminal domain, GST tagged human

SIGMA/SRP2120 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: POLRA; RPB1; RPO2; RPOL2; hRPB220

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SRP2120-10UG	10 µg	$457.00 1/EA	Add To Favorites

Immunoblotting RNA Polymerase II, C-terminal domain, GST tagged Cat. No. SRP2120: RNA Polymerase II, C-terminal domain, GST tagged was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).

Properties
Descriptions
Safety Info.
Basic Data

assay	≥70% (SDS-PAGE)
biological source	human
color	clear colorless
form	frozen liquid
mol wt	~68.1 kDa
NCBI accession no.	NM_000937
packaging	pkg of 10 μg
recombinant	expressed in E. coli
shipped in	dry ice
storage condition	avoid repeated freeze/thaw cycles
storage temp.	−70°C
UniProt accession no.	P24928

Biochem/physiol Actions:	The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo: IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongation complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.
Biochem/physiol Actions:	The CTD works as a binding scaffold for nuclear factors through its phosphorylation. It is suggested to be also involved in chromatin structure modification, DNA damage/repair, protein degradation and synthesis, RNA degradation, snRNA (small nuclear RNA) modification, and snoRNP (small nucleolar ribonucleoprotein) biogenesis.
Physical form:	Clear and colorless frozen liquid solution
Preparation Note:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥70% (SDS-PAGE)
Storage Temp.	−70°C
UNSPSC	12352202

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