LRH-1, GST tagged human
SIGMA/SRP2145 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F-2
Product Type: Chemical
assay | ≥80% (SDS-PAGE) |
biological source | human |
color | clear colorless |
concentration | 500 μg/mL |
form | frozen liquid |
mol wt | ~83 kDa |
NCBI accession no. | NM_003822 |
packaging | pkg of 10 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage temp. | −70°C |
UniProt accession no. | O00482 |
Biochem/physiol Actions: | LRH-1 (NR5A2) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes. LRH-1 is a mammalian homologue of Drosophila fushi tarazu factor (dFTZ-F1) and structurally belongs to the orphan nuclear receptor superfamily. LRH-1 can recognize the DNA sequence 5′-AAGGTCA-3′, the canonical recognition motif for steroidogenic factor 1 (SF-1). Enhancer II (ENII) is one of the critical cis-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hB1F. The role LRH-1 and dosage-sensitive sex reversal, adrenal hypoplasia congenital critical region on the X chromosome, gene 1 (DAX-1) in the regulation of StAR gene expression in human granulosa cell tumor cells has been investigated. LRH-1, is expressed in granulosa cells and has been shown to synergize with the cAMP signaling system to regulate the gonadal type II aromatase promoter in transient transfection assays. Observations support a direct role for LRH-1 in the induction of the progesterone but not the estrogen biosynthetic pathway during granulosa cell differentiation. The human nuclear receptor liver receptor homolog 1 (hLRH-1) plays an important role in the development of breast carcinomas. results indicate that hLRH-1′s control of gene expression is mediated by phospholipid binding, and establish hLRH-1 as a novel target for compounds designed to slow breast cancer development. |
Physical form: | Clear and colorless frozen liquid solution |
Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥80% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352202 |