CAR-LBD (101-348), GST tagged human
SIGMA/SRP2147 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: CAR; CAR1; MB67; MGC150433; MGC97144; MGC97209
Product Type: Chemical
Immunoblotting CAR (101-348), GST tagged Cat. No. SRP2147: CAR (101-348), GST tagged was separated on SDS-PAGE using 1-3 μg protein (left); and a protein marker (right).
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| color | clear |
| colorless | |
| concentration | 100 μg/mL |
| form | frozen liquid |
| mol wt | ~55.3 kDa |
| NCBI accession no. | NM_005122   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q14994 |
| Biochem/physiol Actions: | It forms a complex with phosphatase 2A, heat shock protein 90 (Hsp90) and cytosolic CAR retention protein in the cytoplasm. On activation, it translocates to the nucleus and modulates the expression of genes associated with drug metabolism. The protein regulates transforming growth factor-β (TGF-β) signaling. |
| Biochem/physiol Actions: | The constitutive androstane receptor (CAR) was identified as a member of the orphan nuclear hormone receptor family in 1994. Although constitutively active, it can be further activated by ′phenobarbitol-like′ compounds, the most potent being the synthetic compound TCPOBOP. Upon activation, CAR regulates the xenobiotic drug metabolizing enzymes, cytochrome P450s. There are several overlapping functions between the nuclear receptors PXR and CAR. Recently, CAR, as well as PXR, have been shown to place a role in bile acid clearance and cholestatic liver injury. |
| General description: | The gene encoding this protein is localized on human chromosome 1q23.3. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |