APRIL human
SIGMA/SRP3008 - recombinant, expressed in Hi-5 Insect cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: A Proliferating-
Product Type: Chemical
assay | ≥95% (HPLC) |
≥95% (SDS-PAGE) | |
biological source | human |
color | white to off-white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 16.3 kDa |
packaging | pkg of 10 μg |
recombinant | expressed in Hi-5 Insect cells |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | O75888 |
Biochem/physiol Actions: | APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues. |
General description: | APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues. 11 |
Physical form: | Lyophilized from 20 mM Sodium Acetate, pH 7.5 + 500 mM NaCl. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |