Beta D-4 human
SIGMA/SRP3013 - recombinant, expressed in E. coli, ≥98% (HPLC), suitable for cell culture
Synonym: Beta-defensin 104; DEFB104A
Product Type: Chemical
antibiotic activity spectrum | fungi |
mycobacteria | |
viruses | |
assay | ≥98% (HPLC) |
biological source | human |
color | white to off-white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mode of action | cell membrane | interferes |
mol wt | 6.0 kDa |
packaging | pkg of 20 μg |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
suitability | suitable for |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | Q8WTQ1 |
Biochem/physiol Actions: | Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues. |
General description: | Chemical structure: peptide |
General description: | Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human b-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues. |
Physical form: | Lyophilized from 10 mM Acetic Acid. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP |
Symbol | GHS07 |
Signal word | Warning |
Hazard statements | H315 - H319 |
Precautionary statements | P302 + P352 - P305 + P351 + P338 |
Hazard Codes | Xi |
Risk Statements | 36/38 |
Safety Statements | 26 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |