BDNF human
SIGMA/SRP3014 - Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture
Synonym: Brain Derived Neurotrophic Factor
Product Type: Chemical
BDNF induced differentiation of human SH-SY5Y neuroblastoma cell line into a homogeneous population of cells with neuronal morphology. Human SH-SY5Y cells seeded on Collagen IV (Cat. No. C7521) coated plates, were treated with 10 μM Retinoic acid (Cat. No. R2625) for several days, followed by the addition of BDNF (Cat. No. B3795 or SRP3014). (A)-cells treated with Retinoic acid only. (B) Cells treated with Retinoic acid and 100 ng/mL BDNF for 3 days.
SDS-PAGE BDNF human (Cat. No. SRP3014) was assessed on SDS-PAGE.
Dose response activity of BDNF as measured by its ability to support proliferation of retinoic acid treated SH-SY5Y human neuroblastoma cells Human SH-SY5Y cells seeded on Collagen IV (Cat. No. C7521) coated plates, were treated with 10 μM Retinoic acid (Cat. No. R2625) for 5 days, followed by the addition of BDNF (Cat. No. B3795 or SRP3014). Viability was measured using a resazurin reagent after 72 hours.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | ≤1.00 EU/μg endotoxin, tested |
| mol wt | 27.0 kDa |
| packaging | pkg of 10 μg |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P23560  |
| Analysis Note: | The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y. |
| Biochem/physiol Actions: | Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B). BDNF also plays an important role in vascular function and participates in angiogenesis. It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus. |
| General description: | The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |