BMP-6 human
SIGMA/SRP3017 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: VGR
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 26.2 kDa |
| packaging | pkg of 10 μg |
| potency | 0.03-0.06 μg/mL ED50 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P22004   |
| Biochem/physiol Actions: | BMPs (bone morphogenetic proteins) were initially identified from demineralized bone extracts capable of stimulating endochondral osteogenesis at extraskeletal sites in vivo. BMP6 is thought to be involved in early embryogenesis. In hepatocellular carcinoma (HCC) the expression of this protein is suppressed due to hypermethylation, and this is linked with poor patient outcomes. This protein is also down-regulated due to CpG island methylation in CRC (colorectal carcinoma), although it is not yet been found to be linked with tumor progression or prognosis. BMP6 is involved in chondrocyte differentiation, and in fetal kidney and urinary system development. It is thought to be involved in ovulation, where it elevates the accumulation of neutrophils in ovulatory follicles and reduces the influence of protease inhibitors. |
| General description: | BMP6 (bone morphogenetic protein 6) is one of the BMPs which are secreted signaling proteins belonging to the transforming growth factor-β (TGF-β) superfamily. This gene is localized to human chromosome 6p24.1. Recombinant human BMP-6 is a 26.2kDa homodimeric glycoprotein consisting of two 117 amino acid subunits, which correspond to amino acid residues 397 to 513 of the full-length BMP-6 precursor. |
| Other Notes: | VSSASDYNSS ELKTACRKHE LYVSFQDLGW QDWIIAPKGY AANYCDGECS FPLNAHMNAT NHAIVQTLVH LMNPEYVPKP CCAPTKLNAI SVLYFDDNSN VILKKYRNMV VRACGCH |
| Physical form: | Lyophilized from 10 mM Tris, pH 9.0. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |