EGF human
SIGMA/SRP3027 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: Epidermal Growth Factor; URG; Urogastrone
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 6.2 kDa |
| packaging | pkg of 500 μg |
| potency | ≤0.1 ng/mL |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01133   |
| Application: | EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells). |
| Biochem/physiol Actions: | EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non- small cell lung adenocarcinoma. It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. It controls cell differentiation and neurotrophic processes. |
| General description: | The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27. The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in kidney, salivary gland, cerebrum and prostate. Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Sequence: | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |