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FGF-basic human

SIGMA/SRP3043 - Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym: Catabolin; Endogenous Pyrogen (EP); Leukocyte Endogenous Mediator (LEM); Lymphocyte-activating factor (LAF); Mononuclear Cell Factor (MCF)

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP3043-50UG 50 µg
$428.00
1/EA
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assay ≥95% (HPLC)
  ≥95% (SDS-PAGE)
biological source human
color white to off-white
form lyophilized
impurities <0.1 EU/μg endotoxin, tested
mol wt 17.2 kDa
packaging pkg of 50 μg
potency ≥1x10^7 units/mg
recombinant expressed in E. coli
shipped in wet ice
storage temp. −20°C
technique(s) cell culture | mammalian: suitable
UniProt accession no. P09038 
Application: FGF-basic has been used to supplement serum-free DMEM during culture of HeLa cells.
Biochem/physiol Actions: FGF-basic (fibroblast growth factor), also referred to as FGF2, activates the ERK (extracellular signal-regulated kinase) cascade and facilitates self-renewal of hESCs (human embryonic stem cells). FGF2 positively regulates cell survival and/or proliferation. It is found to negatively regulate BMP (bone morphogenetic protein) signaling, a pathway that induces non-neuronal differentiation.
General description: FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.
Physical form: Lyophilized from 5 mM Tris, pH 7.6 + 150mM NaCl.
Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8° C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 5% Trehalose) and store in working aliquots at -20° C to -80° C.
Sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95% (HPLC); ≥95% (SDS-PAGE)
Storage Temp. −20°C
UNSPSC 12352202

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