Granulocyte-Macrophage Colony-Stimulating Factor human
SIGMA/SRP3050 - GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
Synonym: CSF-2; MGI-1GM; pluripoietin-a
Product Type: Chemical
SDS-PAGE Granulocyte-Macrophage Colony-Stimulating Factor human (Cat. No. SRP3050) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of GM-CSF that elicits a 50% attachment factor in cell based Bioassay.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | ≤1 EU/μg endotoxin, tested |
| mol wt | 14.6 kDa |
| packaging | pkg of 20 μg |
| potency | ≤0.1 ng/mL |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04141  |
| Application: | Granulocyte-Macrophage Colony-Stimulating Factor human has been used in the medium for plating of the cells. It has been used in culture medium for the differentiation of human peripheral blood mononuclear cells. |
| Biochem/physiol Actions: | GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration and enhances the functional activity of the mature end-cells. |
| General description: | GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. Recombinant human GM-CSF is a 14.6kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. The human and murine molecules are species-specific and exhibit no cross-species reactivity. |
| Other Notes: | MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
| Physical form: | Lyophilized from 10 mM Sodium Citrate, pH 3.5. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |