IL-10 human
SIGMA/SRP3071 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: B-TCGF; CSIF; TGIF
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | human |
color | white to off-white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 18.6 kDa |
packaging | pkg of 10 μg |
potency | ≤2.0 ng/mL ED50 |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P22301 |
Application: | IL-10 human has been used to study the effect of IL-10 on endothelial nitric oxide synthase and nitric oxide in African American human umbilical vein endothelial cells. It has also been used to study the effect of IL-10 on leukotriene (LT) A4 hydrolase and LTC4 synthase protein expression in and secretion from, an inflamed porcine endometrium. |
Biochem/physiol Actions: | IL-10 (interleukin 10) is an anti-inflammatory cytokine. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF (tumor necrosis factor)-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. Polymorphism in IL-10 at -1082A/G is associated with diabetic nephropathy. |
General description: | IL-10 (interleukin 10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. The gene is mapped to human chromosome 1q31-1q32. Recombinant human IL-10 is an 18.6kDa protein of 161 amino acid residues. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. |
Physical form: | Lyophilized from 5 mM Sodium Phosphate, pH 7.2. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |