Interleukin-2, human
SIGMA/SRP3085 - Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
Synonym: Aldesleukin; IL-2; T-cell growth factor (TCGF)
Product Type: Chemical
| assay | ≥95% (HPLC) |
| biological source | human |
| form | (Lyophilized with no additives from a 0.2µ filtered solution) |
| impurities | ≤1.00 EU/μg endotoxin, tested |
| mol wt | 15.5 kDa |
| packaging | pkg of 50 μg |
| potency | ≤0.5 ng/mL ED50 |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P60568  |
| Application: | • IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform. • It has been used for the production of tumor-specific cytotoxic T lymphocytes. • It has been used for the proliferation of T lymphocytes. |
| Biochem/physiol Actions: | IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways. |
| General description: | IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. The gene is mapped to human chromosome 4q27. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 1 week. For extended storage, freeze in working aliquots. It may be advisable to centrifuge the vial prior to reconstitution. |
| Sequence: | MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |