IL-3 human
SIGMA/SRP3090 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: HCGF; MCGF (Mast cell growth factor); Multi-CSF; P-cell stimulation factor
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 15.0 kDa |
| packaging | pkg of 10 μg |
| potency | ≤0.1 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P08700   |
| Biochem/physiol Actions: | IL3 (interleukin 3) promotes the survival, proliferation and differentiation of haematopoietic progenitor cells. It also functions as an activator of basophils thus, resulting in significant increase in antigen and IgE-mediated release of cytokines and other mediators. Certain variants in this gene confer protection against malaria attack, and thus, this gene might play a role in the pathophysiology of falciparum malaria. SNP (single nucleotide polymorphism) rs40401 in this gene and smoking have a positive correlation with asthma in Japanese women. IL3 is involved in brain development and has a direct influence on the proliferation and survival of neural progenitor cell population, thereby also controlling human brain volume. |
| General description: | IL3 (interleukin 3) is a haematopoietic growth factor, the gene for which is localized to human chromosome locus 5. Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Sequence: | APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |