IL-5 human
SIGMA/SRP3095 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: BCDFII; EDF
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | human |
color | off-white to yellow |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 26.0 kDa |
packaging | pkg of 10 μg |
potency | ≤0.25 ng/mL ED50 |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P05113 |
Biochem/physiol Actions: | IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains. |
General description: | IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. Elevated levels of IL-5 lead to Eosinophilia, which may result in the induction of asthma and other allergic diseases. Human and murine IL-5 is cross-species reactive. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains. |
Physical form: | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium glycinate, pH 8.5 |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |